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Optimizing the screening stringency by 3-AT
| Plasmids for Transformation | Concentration of 3-AT/mM | Number of colonies on the basic plate | Comment |
|---|---|---|---|
| pBT3-SUC-APP+pPR3-N | 2.5 | 8 | Strong background, unsuitable for screening |
| 5 | 7 | Strong background, unsuitable for screening | |
| 7.5 | 3 | Low background, suitable for screening | |
| 10 | 0 | No background, maybe a little stringent for screening | |
| 20 | 0 | No background, too stringent for screening |
