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. 2015 Mar 1;8(3):2829–2834.

Figure 3.

Figure 3

miR-570 represses KLF9 expression through targeting its 3’-UTR. A. Prediction of miR-570 binding sites in the 3’UTRs of human KLF9 gene by miRWalk software. Potential binding site was highlighted in bold. B. Luciferase reporter assays in A549 cells. Cells were transfected with 200 ng of wild-type or mutant 3’-UTR-reporter constructs together with 50 nM of miR-570 precursor or negative control (NC). C, D. Western blot analysis of KLF9 in A549 and NCI-H358 cells transfected with miR-570 mimics or negative control (NC). E. Representative protein levels of KLF9 in human lung cancer tissues (Tumor) and adjacent non-cancerous normal tissues (Normal).