Figure 2.

CD9 inhibits the growth of TE-1 cells. A. Western blot analysis of CD9 was performed on TE-1 cells with transfection with siRNA-NC or siRNA-CD9. CD9 protein level was higher in siRNA-NC group than that in siRNA-CD9 group. α-Tubulin was used as an internal control to ensure equal loading. B. TE-1 cells were analysis for their ability to form colonies via colony formation assay. Cells were transfected with siRNA-NC or siRNA-CD9 and incubated for 10 days. The figure showed one representative experiment repeated for three times with the similar results. C. The relative number of colonies formed after 10 days’ culture in siRNA-CD9 group was significantly more than that in siRNA-NC group (P < 0.05). D. Downregulation of CD9 increased the size of TE-1 cell size compared with the controls (P < 0.05).