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. 2015 May 21;5:10484. doi: 10.1038/srep10484

Figure 5. Localization of TcCP30 protein in elytral cuticle during development.

Figure 5

Cryosections (~12 μm) of 4 and 5 d-old pupae and 0 to 1 d-old adults were incubated with the anti-TcCP30 antibody. Anti-TcCP30 antibody was detected by Alexa Fluor 546-conjugated anti-rabbit IgG antibody (red). FITC-conjugated chitin-binding probe (FITC-CBD) was used to stain cuticular chitin (green). Nuclei were stained with TO-PRO3 (blue). In merged images, yellow: TcCP30 and chitin, purple: TcCP30 and nuclei. P4, day 4 pupa; P5, day 5 pupa; A0, day 0 adult; A1, day 1 adult. E, elytron; D, dorsal elytral cuticle; V, ventral elytral cuticle; DE, dorsal elytral layer of epithelial cells; VE, ventral elytral layer of epithelial cells; H, hindwing; T, trachea; P, pupal cuticle. Scale bar = 20 μm.