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. 2015 May 21;5:10484. doi: 10.1038/srep10484

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Ultra-thin sections (~90 nm) of pharate adults (day 5 pupae) that had been injected with dsTcCP30 or dsTcVer (200 ng per insects) at late larval stage were incubated with anti-TcCP30 antibody. The TcCP30 antibody was then detected using goat anti-rabbit IgG conjugated to 10 nm gold particles. TcCP30 protein is present in both horizontal chitin laminae and vertical PCFs of dorsal elytral cuticle of dsTcVer-treated control insects (left panels). Following RNAi for TcCP30, the abundance of gold particles is decreased in TcCP30-deficient insects (right panels) compared to dsTcVer-treated control insects. APMP, apical plasma membrane protrusions; EN, envelope; EP, epicuticle; PCF, pore canal fibers. Scale bar = 500 nm.