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Sequential ND-FISH experiments on chromosomes of wheat-rye 1R disomic addition line 14T-71 performing with Oligo-1162 (green), Oligo-pTa71-2 (green), Oligo-pSc119.2-1 (green) and Oligo-pTa535-1 (red) as probes. a Oligo-1162 signals on chromosomes of line 14T-71. b Oligo-pTa71-2 signals on the same cell in (a). c Oligo-pSc119.2-1 and Oligo-pTa535-1 signals on the same cell in (a) and (b). Chromosomes were counterstained with DAPI (blue). Scale bar 10 μm.
