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. 2014 Oct 24;33(1):425–432. doi: 10.3892/or.2014.3569

Figure 2.

Figure 2

CD271+ cells express high levels of stem-related gene NANOG and EMT markers. (A) CD271 expression in KYSE70 was detected by flow cytometry. Of the cells 7.5% intensely expressed CD271. (B) The purity of sorted CD271+ and CD271− cells was 99.41 and 98.36%, respectively. (C) Quantitative real-time PCR analysis of CD271 expression in sorted CD271+ and CD271− cells. In the two subpopulations, relative expression of (D) NANOG, (E) Fibronectin (FIBRO), (F) Vimentin (VIM) and (G) apoptosis genes BAK, Caspase 3 and Caspase 9 was analyzed by qPCR.