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CD271 expression is regulated by DNA methylation. (A) CD271 promoter region. The analyzed CpG dinucleotides are underlined. Primers used for bisufilte genomic sequencing are in bold. Methylation status of 31 CpG sites in the promoter region of KYSE70 cells treated (B) without or (C) with demethylation agent 5-aza. (D) qPCR analysis of CD271 expression in KYSE70 cells treated with or without 5-aza.
