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. Author manuscript; available in PMC: 2016 May 19.
Published in final edited form as: Immunity. 2015 May 19;42(5):953–964. doi: 10.1016/j.immuni.2015.04.016

Figure 3. Upregulation of IL-1β and IL-18 production in HFD-induced skin lesions.

Figure 3

(A-B) Keratinocytes, CD11c+ macrophages and γδ T cells were separated by a flow sorter from either normal skin tissues or HFD-induced lesional skin tissues. Relative levels of IL-1β mRNA (A) and IL-18 mRNA (B) were determined by quantitative real-time PCR (*, p<0.05; **, p<0.01).

(C-D) Keratinocytes, CD11c+ macrophages and γδ T cells were separated by a flow sorter from feltwheel-induced lesional skin tissues from lean or obese mice. Relative levels of IL-1β mRNA (A) and IL-18 mRNA (B) were determined by quantitative real-time PCR (*, p<0.05).

(E-F) Measurement of IL-1β (E) and IL-18 (F) by ELISA in supernatants of primary keratinocytes or CD11c+ macrophages stimulated with designated conditions for 24 hours (LPS, 100ng/ml, FAs, 200μM palmitate) (*, p<0.05; **, p<0.01).

Data are shown as mean ± SEM and representative of at least three experiments. See also Table S1 and Figure S2.