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Effect of GO and NDEA on total AKT, p-AKT (Thr308), p-AKT (Ser473) and p-AKT (Tyr450) protein contents. A: A representative immunoblot. B: Data presented the expressions of total AKT, p-AKT (Thr 308), p-AKT (Ser473) and p-AKT (Tyr450) as percentage of control group (mean ± SD) in triplicate. The protein levels were quantified with β-actin as an internal control. *P<0.05, **P<0.01, compared with control group; #P<0.05, ##P<0.01, compared with NDEA group; &, P<0.05, &&, P<0.01.
