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. 2015 May 22;15:33. doi: 10.1186/s12896-015-0144-x

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© Nakagawa et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Schematic design of multiple gene targeting using all-in-one FokI-dCas9 vector. (A) The genomic context around the targeted loci. The target sites are located in exon 3 of Reg3b and Reg3g genes. (B) The target sequence and the constructed vectors. The target sequence of each gRNA is indicated by colored bases. The PAM sequence is underlined.