Fig. 2.
Silencing of ARID1A promotes HCC cell migration and invasion. a shows cell migration ability was increased in ARID1A knockdown Huh7 cell line, while (b) exhibits its effect on invasion. For migration/invasion assay cells were cultured in the upper chamber of transwell for 24 h/48 h respectively. c We examined epithelial–mesenchymal transition (EMT) associated proteins, including E-Cadherin, N-Cadherin, Fibronectin and Vimentin in ARID1A knockdown Huh7 cells. d The expression level of both ARID1A and CDH1 (E-Cadherin) was evaluated by qPCR in 36 paired HCC specimens collected from Zhongshan Hospital, Xiamen, China. Correlation coefficient (R 2) of both proteins was calculated as log2-transformed expression between normal and cancer tissues