Figure 2.

Rapid 3-D delineation of nuclei in DAPI stained human cancer cells. Workflow in a) refers to example output images at mid-optical section of a z-stack: b) original image, c) preliminary mask of nuclei after background removal, d) output of 3-D radial symmetry transform obtained from the original image, e) seeds superimposed onto the original image, and f) delineated nuclei after final seed placement followed by seeded watershed segmentation.