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. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: Cell Rep. 2015 Apr 9;11(3):344–350. doi: 10.1016/j.celrep.2015.03.032

Figure 3. Knockdown of Esrra Expression by shRNA in Cortex Produces Region-Specific Behavioral Deficits.

Figure 3

(A) Body-weight gain in female C57BL6/J mice on a HFD after AAV delivery into cingulate (n = 10 scramble and 9 shRNA-Esrra), orbitofrontal (n = 9 scramble and 10 shRNA-Esrra), or mPFC (n = 9 scramble and 11 shRNA-Esrra).

(B) Intake of a HFD after Esrra knockdown in mPFC.

(C) A separate cohort of mice received AAV into mPFC and was tested for total lever presses and reward earned.

(D and E) Mice from (A) were tested for home-cage grooming (D) and immobility in the forced-swim test (E).

Data presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001 indicate significant differences between groups (ANOVA [A] or Student’s t test [B–E]). See also Figure S2.