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. Author manuscript; available in PMC: 2015 May 21.
Published in final edited form as: Free Radic Biol Med. 2014 Feb 7;69:348–356. doi: 10.1016/j.freeradbiomed.2014.01.038

Fig. 4.

Fig. 4

Heparin binding capacity of secreted EC-SOD. BMMs were derived from wild-type animals and incubated in the absence (−LPS) or presence (+LPS) of LPS. The cell culture medium was collected for analysis by affinity chromatography using heparin–Sepharose. The starting material (medium), the column flowthrough (FT), and the eluate were analyzed by immunoprecipitation and Western blotting. The presence of intact and cleaved EC-SOD is indicated on the left. The control samples represent absorption of EC-SOD present in a mouse lung homogenate. The intensity and ratio of the EC-SOD subunits were evaluated by using ImageJ.