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. Author manuscript; available in PMC: 2015 May 21.
Published in final edited form as: Comput Methods Programs Biomed. 2008 Jul 21;92(1):54–65. doi: 10.1016/j.cmpb.2008.06.002

Figure 1. Two- and three-color Fluorospot assays detect various cell phenotypes.

Figure 1

A) 2×103 Th1 cells per well were restimulated in a Fluorospot assay for MIP-1α (red, Cy3) and IFN-γ (green, Cy2). B) 2×103 Th1 and 7×103 Th2 cells per well were restimulated in a Fluorospot assay for IL-4 (red, Cy3) and IFN-γ (green, Cy2). C) CD4 T cells expressing a range of cytokine secretion phenotypes were restimulated in a Fluorospot assay for IL-5 (red, Cy3), IL-2 (green, Cy2) and IFN-γ (blue, Cy5). The top three rows show single-channel images for red, green and blue, and the bottom rows show the composite images.