Figure 1.

Process design. (A) Summary of overall scheme: paucicellular samples are harvested and captured on the chip without preprocessing. Following on-chip fixation, permeabilization, and immunostaining, the chip is imaged and cytometry is carried out with an in-house image process algorithm. (B) Photograph of lymphocyte capture chip attached to a microslide, showing inlet, debris filter, and capture area, which contains four arrays of 20 × 300 single-cell capture sites.