Figure 2.

Validation of on-chip capture and imaging. (A) DB cells dual-labeled with Hoechst and anti-CD45-APC, and captured and imaged on-chip. Capture sites are butterfly-shaped, staggered, and customized for lymphocyte size-based capture. Scale bar: 25 µm. (B) Capture efficiency of DB and Daudi cells is greater than 90% when 10, 100, or 1,000 lymphoma cells (nominal numbers) were introduced to the chip. The counted cell number is displayed as mean ± s.d. from quadruplicate measurements. The nominal cell number is displaced as mean ± Poisson error. (C) Optimization of flow rate based on capture efficiency of DB cells. (D) Proposed workflow for clinical diagnosis using image analysis.