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. 2015 Apr 26;5(8):834–846. doi: 10.7150/thno.12040

Fig 2.

Fig 2

Biorecognition of Fab'-MORF1 and P-MORF2 at the surface of Raji B-cells. Fab'-MORF1 was labeled with rhodamine (red) and P-MORF2 with FITC (green). Trans: images under transmitted light, R: red channel, G: green channel. (A) Top panel, Fab'-MORF1 (1 μM) bound to the surfaces of B-cells. Bottom panel, pretreatment with 1F5 mAb (10 μM) followed by Fab'-MORF1 (1 μM). (B) Top panel, consecutive exposure to Fab'-MORF1 (1 μM) and P-MORF2 (1 μM; MORF eqv.). Bottom panel, polymer grafted with scrambled MORF2 (P-scMORF2; 5 μM MORF eqv.) failed to bind to the surfaces of the MORF1-decorated cells; scMORF2: 5'-GTATCCTTATTCCACGTTCATTTGT-3'. Note: P-MORF2 and P-scMORF2 used in this experiment only contained ~5 oligonucleotides per chain.