Fig 7.

Drug-free nanotherapeutic induces apoptosis of mantle cell lymphoma (MCL) cells from patients. Two samples were from lymph node biopsies (patients 1 and 2) and two from the peripheral blood (patients 3 and 4). Percent apoptotic cells was analyzed by annexin V binding and quantified with flow cytometry. Nanomedicine, Fab'-MORF1 followed by P-MORF2; Rituximab, rituximab followed by goat anti-mouse secondary Ab; 1F5 mAb, 1F5 followed by goat anti-mouse secondary Ab. Time interval between treatments was 1 h (unless otherwise indicated). Incubation time was 24 h. Fab' equivalent concentrations of treatments were 0.5 μM (for patients 1, 2 and 3) and 1 μM (for patients 3 and 4). Data are presented as mean ± SD (n = 2 or 3). Statistics was performed by student's t test (*: p < 0.05). All treatment groups had significantly higher apoptotic indices when compared to the untreated cells.