Table 3.
Summary of inflammatory cell counts, LDH, total protein, and inflammatory mediators at 24 h after intratracheal instillation of CoCl2
| BALF measure | VEHsaline | CoCl2 (μg/rat)a | ||
|---|---|---|---|---|
| 69 (31)b | 173 (79) | 693 (315) | ||
| Total cells (× 105) | 13.65 ± 2.63 | 24.68 ± 5.12 | 28.35 ± 3.96 | 18.30 ± 1.54 |
| Macrophages (× 105) | 11.84 ± 1.36 | 14.12 ± 1.81 | 13.66 ± 0.80 | 2.92 ± 0.746** |
| Neutrophils (× 105) | 1.76 ± 1.38 | 10.48 ± 3.72* | 13.74 ± 3.77** | 11.70 ± 2.75*** |
| Eosinophils (× 105) | 0.02 ± 0.02 | 0.06 ± 0.04 | 0.95 ± 0.56 | 3.63 ± 0.81** |
| Lymphocytes (× 105) | 0.05 ± 0.01 | 0.10 ± 0.04 | 0.04 ± 0.02 | 0.02 ± 0.02 |
| LDH (fold vs control) | 1.00 ± 0.12 | 3.08 ± 0.53* | 6.65 ± 0.78*** | 15.05 ± 0.18*** |
| Total protein (μg/mL) | 270 ± 47 | 341 ± 67 | 700 ± 165 | 5051 ± 917*** |
| CINC-3 (pg/mL) | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
| IL-6 (pg/mL) | 0.00 ± 0.00 | 0.00 ± 0.00 | 39.2 ± 39.2 | 734.6 ± 152.3*** |
| Eotaxin (pg/mL) | 10.76 ± 3.27 | 30.94 ± 16.60 | 133.1 ± 49.2 | 699.8 ± 38.1*** |
| IL-13 (pg/mL) | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
aCo ions were prepared by dissolving CoCl2 in saline and treated at equivalent dose for Co of CoO NPs (40, 100, and 400 μg/rat)
bParenthesis represents the Co ions mass for CoCl2 which is equivalent Co ions doses of CoO NPs
Data were presented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001, statistically different from vehicle control (VEHsaline)
VEHsaline, sterile 0.9 % saline was used as a vehicle control for CoCl2; LDH, lactate dehydrogenase; CINC-3, cytokine-induced neutrophil chemoattractant-3