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. 2015 May 21;5:9758. doi: 10.1038/srep09758

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Copyright © 2015, Macmillan Publishers Limited

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Deep-transcriptome sequencing of purified cardiovascular lineages differentiated from hESC. (a) Schematic representation of the sampling during cardiovascular differentiation from hESCs. (ES: embryonic stem cell; see other abbreviations in the main text; MCP, multipotential cardiovascular progenitors; CM, cardiomyocytes; SM, smooth muscle cells; EC, endothelial cells). (b) Summary of the deep-sequencing results. (c) Expression of the lineage marker genes quantitated by the normalized FPKM. Types of markers are color-coded. Blue: pluripotency markers; dark orange: mesoderm and early cardiac markers; red: cardiomyocyte markers; green: smooth muscle cell markers; purple: endothelial cell markers. (d) qRT-PCR validation of the marker gene expression. (e) Principle component analysis using FPKM of all the sequenced genes of the 5 cell types.