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. Author manuscript; available in PMC: 2015 May 21.
Published in final edited form as: Mol Carcinog. 2011 Aug 18;51(9):734–745. doi: 10.1002/mc.20840

Figure 3. COX-2 modulates UVB-induced β-catenin signaling.

Figure 3

(A, B) 308 cells were pretreated with DMSO vehicle control [D] or the indicated doses of NS398 for 1 hr. Cells were then sham irradiated or exposed to 500 J/m2 UVB and incubated for 8 hrs. (A) Cell culture media was collected and PGE2 levels were measured by competitive ELISA. (B) Protein lysates were probed for unphospho-β-catenin, total β-catenin and GAPDH. Representative blots are shown and numbers indicate fold change of proteins normalized to GAPDH. (C) COX-2 WT and KO MEFs were sham irradiated or exposed to 500 J/m2 UVB and incubated for 8 hrs. Real time RT-PCR was performed to determine expression levels of AXIN2 mRNA. **, P < 0.01.