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. 2015 Apr 30;4:13. doi: 10.1186/s40164-015-0007-0

Figure 5.

Figure 5

Measurement of immunosuppressive mediators. (A) iNOS production in host colon. (B) Phosphorylated STAT 5A/B levels in the host spleen and colon. Lethally irradiated BALB/c host mice were given intravenous injections of 2 × 10^6 TCDBM cells from C57BL/6 donors with or without 0.25 ×10 ^6 CD4+ T cells from donors and 1 × 10 ^6 hMSCs. (A) Host colon was harvested 6d after transplantation and iNOS expression detected by western blotting method. Each lane contains 50 ug of protein. B-actin served as loading control. Intensity of bands is expressed in INT × 10^4/mm^2 units and analyzed using The Discovery series Quantity 1D Analysis software, Biorad Laboratories, Inc., USA. *p = 0.0215. Data were combined from 3 independent experiments with 2-3 mice in each experiment. (B) For testing the levels of phosphorylated STAT 5A/B in the host spleen and colon, tissues were harvested 6d after transplantation and levels were determined by using ELISA. (***p = 0.0007). Data were combined from 2 independent experiments with 3-4 mice in each experiment.