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. Author manuscript; available in PMC: 2015 May 21.
Published in final edited form as: Bone. 2013 Dec 4;60:148–161. doi: 10.1016/j.bone.2013.11.020

Figure 6. Analysis of endochondral bone formation. Panels a-l.

Figure 6

In situ hybridization of adult lumbar spine for Prp (PTH/PTHRP receptor) (panels a-f; a-d, dark field; e, f, bright field) and Ihh (Panels g-l; g-j, dark field; k, l, bright field). Original magnification is given to the left. Panels m-p. Immunohistochemistry for collagen 2a1 and collagen 10a1 in newborn wildtype and MEm1/m1 mice. Coronal sections of L4 vertebrae in paraffin. Panels m, n: collagen 2a1 stain; Panels o and p: collagen 10a1 stain. Collagen 2 is expressed throughout the early vertebral body and IVD, while collagen 10a1 is limited to the mineralizing core of the vertebral body.