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. 2015 Apr 23;54(19):3100–3109. doi: 10.1021/acs.biochem.5b00277

Figure 2.

Figure 2

(a) Comparison of binding/uptake of Cy5** and BLM disaccharide–Cy5** conjugate (1) in three cancer cell lines. The cells were treated with 25 μM BLM disaccharide–Cy5** (1) or Cy5** at 37 °C for 1 h, washed with PBS, and fixed with 4% paraformaldehyde. The cell nuclei were stained with DAPI. Fluorescence imaging was carried out after a 2 s exposure. (b) Quantification of the binding/uptake of BLM disaccharide–Cy5** and Cy5** in three sets of matched cancer and normal cell lines. The cells were treated with 25 μM dye (conjugate) and irradiated for 2 s prior to imaging.