Figure 6.

(a) Effect of lysine linkers on the internalization of BLM disaccharide–Cy5** conjugates (4 and 5) on binding/uptake in four cell lines. The cells were treated with 25 μM BLM disaccharide–Cy5** analogues at 37 °C for 1 h, washed with PBS, and fixed with 4% paraformaldehyde. Fluorescence imaging was carried out with a 2 s exposure time. (b) Quantification of the binding/uptake of 25 μM dye conjugates in different cancer cells and irradiated for 2 s prior to imaging.