Figure 3.

The effects of nuclease treatment on the binding of mAbs to MPs. MPs prepared from Jurkat (A) and THP-1 (B) cells were treated with 100 U/ml of DNase (black line) or left untreated (filled peak). MPs were incubated with the mAbs (5 μg/ml) for 1 h at RT followed by staining with R-PE labeled goat anti-mouse IgG. Stained MPs were assayed by flow cytometry. Results are representative of four replicate experiments.