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. 2015 Apr 26;6:1–10.

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© Copyright The Author(s)

First Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.

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Figure 1. — Expression and purification of r-Cam-dis analysis by 4–12% SDS-PAGE gel under non-reducing condition. Samples were run on 4–12% (w/v) Bis-Tris Gel using an Xcell SureLock Mini-Cell at 200V for 30min. The gel was stained with Rapid-Stain. Lane 1: SeeBlue Plus2 Markers; lane 2: soluble fraction of lysates of expressed E. coli BL21 cells by BugBuster reagent (150μg); lane 3: cleaved r-Cam-dis after wash with binding buffer (3μg); lane 4: purified r-Cam-dis after wash with high salt buffer (3 μg). An asterisk (^*) represents the N-terminal amino acid sequence of purified r-Cam-dis containing the five amino acids from the vector (italicized) before the disintegrin sequence, which are shown in bold letters.