. 2015 May 21;11(5):e1005134. doi: 10.1371/journal.pgen.1005134

Table 1. Bacterial strains and plasmids used in this study.

Strain or plasmid	Relevant genotype	Reference or source
E. coli K-12 strains
MG1655	Parental strain	Lab collection
DV901	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ	[51]
DV915	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ ΔiscR::kan	[51]
DV925	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ ΔcyaY	Lab collection
BR667	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ ΔiscU	This study
DV1093	MG1655 MVA⁺	[51]
BR755	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ iscU _I108M ΔyphD	This study
BR756	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ iscU _I108M ΔyphD ΔcyaY	This study
BR757	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ iscU _I108M ΔyphD MVA ⁺::kan	This study
BR763	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ iscU _I108M ΔyphD MVA ⁺::kan Δsuf::cat	This study
BR767	lacIpoZ Δ (Mlu) λ -P_iscR -lacZ iscU _I108M ΔyphD MVA ⁺::kan Δsuf::cat ΔcyaY::spec	This study
PM12.05	MG1655 mal::lacI ^q, ΔaraBAD, lacI’::P _BAD -cat-sacB:lacZ, mini λ tet ^R	[68]
Plasmids
pET22b-CyaY	pET22b expressing CyaY-(his)₆	[85]
pQE-30-IscS	pQE30 expressing (his)₆-IscS	[74]
pET21a-IscU	pET21a expressing IscU-(his)₆	This study
pET21a-IscU_IM	pET21a expressing IscU_I108M-(his)₆	This study