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Cu-ATCUN-sh-buforin complexes promote formation of intracellular ROS in E. coli. Relative fluorescence units were used as a measure of generalized oxidative stress caused by exposing the cells to Cu-ATCUN-sh-buforin complexes ([Cu-ATCUN-sh-buforin] = MIC/4), 10 μm H2O2 or 32 μm Cu2+ ions. The mean ± standard deviation of three independent experiments is shown (*, P ≤0.05; N.S. = not significant).
