Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 May 21;10(5):e0127888. doi: 10.1371/journal.pone.0127888

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Watanabe-Takano et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 4 — (A) Suppression of TGF-β1-induced αSMA expression by knockdown of DA-Raf with DAraf siRNAs. RLE cells were transfected with DAraf siRNAs as well as a control siRNA. Twenty-four hours after the transfection, they were treated with 0.5 ng/ml TGF-β1 for 48 h. The levels of DA-Raf, A-Raf, αSMA, and E-cadherin, as well as β-tubulin as a standard, were analyzed by immunoblotting. (B) Suppression of TGF-β1-induced αSMA expression with DAraf siRNAs. RLE cells were transfected with DAraf siRNAs and treated with 0.5 ng/ml TGF-β1. αSMA expression (red) and nuclei (blue) were detected by fluorescence microscopy. Scale bar, 50 μm. (C) The ratio of αSMA-expressing cells in the analysis of (B). The values are means ± SD of 3 independent experiments. **, P < 0.01 by t test. (D) Elevation of the TGF-β1-suppressed Cdh1 (E-cadherin) mRNA level and suppression of the TGF-β1-induced Acta2 (αSMA) mRNA level with DAraf siRNAs. RLE cells were transfected with DAraf siRNAs and treated with TGF-β1 for 48 h. Relative levels of Cdh1 and Acta2 mRNAs normalized to the Actb (β-actin) mRNA level were determined by real-time PCR. The values are means ± SD of 3 independent experiments. **, P < 0.01 by t test. (E) Dose-dependent induction of αSMA expression by TGF-β1 and its suppression by DAraf siRNAs. RLE cells were transfected with DAraf siRNAs and treated with 0.1–5 ng/ml TGF-β1 for 48 h. The intensities of αSMA and β-tubulin bands on immunoblots were analyzed by densitometry. The graph shows the ratio of αSMA to β-tubulin band intensity against TGF-β1 concentration. The values are means ± SD of 3 independent experiments. a.u., arbitrary units.