Fig 2. Incubation of muscle in 25mM diminishes SIRT1 protein abundance, NAMPT activity, and NAD/NADH ratio after 1 or 2h.

EDL were incubated with 25 mM glucose for 30, 60 or 120 min. Western blot analysis and quantification of representative blots are shown. SIRT1 protein expression (A) NAMPT activity (B) and NAD/NADH (C) were determined as described in the methods section. Results are means + SE (n = 6). *P < 0.05 compared to 30 min incubation with 5.5 mM glucose.