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. 2015 May 22;6:519. doi: 10.3389/fmicb.2015.00519

Figure 4.

Figure 4

Comparison of the sensitivities of the LAMP reaction and conventional PCR in detecting the rcsAgene of K. pneumoniae. Pure genomic DNA extracted from K. pneumoniae ATCC BAA-2146 was serially diluted 10-fold. (A) Turbidity was monitored every 6 s with a Loopamp Realtime Turbidimeter at 650 nm. (B) The reaction result was detected visually by the addition of 1 μl of fluorescent detection reagent to the 25 μl LAMP reaction mixture before the LAMP reaction. (C) PCR products were separated by 2% agarose gel electrophoresis and stained with ethidium bromide. Amplification was performed at 61°C for 60 min. 1, negative control (double-distilled water); 2, 115.0 ng/μl; 3, 11.5 ng/μl; 4, 1.15 ng/μl; 5, 115.0 pg/μl; 6, 11.5 pg/μl; 7, 1.15 pg/μl; 8, 0.115 pg/μl; 9, 0.0115 pg/μl.