FIGURE 2.

High-throughput molecular analysis of microbial communities of the phyllosphere: from direct DNA sequencing to sequencing of prokaryotic and eukaryotic taxonomical markers. The present range of published studies covers a significant number of plant host species in various developmental and spatio-temporal contexts and under the influence of different environmental factors. The articles presented in Supplementary Table S1 represent a selection of studies that were performed in the last years using NGS technologies. Further studies can be found in tables of other recent reviews (Rastogi et al., 2013; Knief, 2014; Müller and Ruppel, 2014). Taken together, these studies give a dynamic view of phyllosphere microbiota biodiversity, metabolisms, and environmental plasticity.