Fig. 1.

Selectivity towards hESC-derived NSCs in vitro. (A) Representative images of areas of hESC-derived neuronal cultures containing neural stem and progenitor cells (top row, red) or mature neurons (bottom row, red), 48 h post-infection with recombinant AAV2, AAV6 or AAV r3.45 vectors expressing GFP (green). Representative examples of infected cells of each type are marked with arrowheads. Scale bar: 100 μm. (B) The percentage of GFP⁺ cells co-staining for nestin or MAP2B was quantified to determine the selectivity of each viral vector. Error bars indicate s.d. (n=3); *P<0.01, **P<0.005 (ANOVA).