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. 2015 May 15;142(10):1885–1892. doi: 10.1242/dev.115253

Fig. 4.

Fig. 4.

Proliferation and differentiation of NSCs induced by CA β-catenin. (A,B) Representative images at low (20×) magnification of the mouse dentate gyrus 3 weeks post-injection of recombinant AAV r3.45 vectors expressing (A) GFP (green) or (B) CA β-catenin-2×FLAG (green). Brain sections were co-stained for NeuN (blue) and EdU (red), and infected cells co-staining for NeuN and EdU are marked with arrowheads. Scale bar: 100 μm. (C) Infected cells (FLAG+, β-catenin condition; GFP+, control condition) co-staining for EdU (a cell division marker), nestin (an NSC marker), Dcx (an immature neuronal marker) and/or NeuN (a neuronal marker) were analyzed to determine the degree to which β-catenin stimulates the proliferation and differentiation of NSCs. Error bars indicate s.d. (n=3); *P<0.001 (ANOVA).