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. 2015 May 15;142(10):1850–1859. doi: 10.1242/dev.123653

Fig. 5.

Fig. 5.

FGF signals are neither required nor sufficient to induce a neural retina character. (A) Fgf8 expression is upregulated within the neural retina at stage 13-14 (stages demarcated by black line). (B-E) Stage 9/10 OVL explants were cultured to approximately stage 21 and analyzed by in situ hybridization on consecutive sections. (B) Stage 9/10, OVL explants generate Fgf8+ (15/15) cells within the retinal domain. (C) In stage 9/10 OVL explants cultured together with Noggin, Fgf8 (0/15) expression was inhibited. (D) In the presence of Noggin and FGF8, stage 9/10 OVL explants still generated FoxG1+ (12/12) telencephalic cells, but did not generate Rax2+ (0/12) or Vsx2+ (0/12) neural retina cells. (E) In stage 9/10 OVL explants cultured together with SU5402, the generation of Rax2+ (12/12) and Vsx2+ (12/12) neural retina cells was not inhibited. No FoxG1 (0/12) expression was observed. Scale bar: 100 µm.