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. 2015 May 15;49(3):236–242. doi: 10.4132/jptm.2015.03.16

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© 2015 The Korean Society of Pathologists/The Korean Society for Cytopathology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — In immunohistochemistry of Ki67 (A–D), EDTA (A) shows intact quality while HCl (B) shows poor quality in a pair of bone marrow sample from the same patient. A similar result is noted in comparison of RDO (C) versus HCl (D). Nuclear staining of cyclin D1 is intact in EDTA (E), while it is not in HCl (F) of a paired bone marrow from the same patient. Nuclear staining of TdT is intact in RDO (G), while it is poor in HCl (H) of a paired bone marrow from the same patient. Cytoplasmic membrane staining of CD138 (I–L) reveals intact quality in all three protocols: EDTA (I), RDO (K), and HCl (J, L). EDTA, ethylenediaminetetraacetic acid disodium salt dehydrate; HCl, hydrochloric acid; RDO, RDO GOLD.