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The decalcification protocols of three methods
| Solution | Processing time (hr) | Processing temperature |
|---|---|---|
| HCl (100%) | 3 | Room temperature |
| EDTA (12.5%) | 3 or 24a | Room temperature |
| RDO (100%) | 0.5–1 | Room temperature |
HCl, hydrochloric acid; EDTA, ethylenediaminetetraacetic acid disodium salt dehydrate; RDO, RDO GOLD.
The processing time of EDTA method was mostly 3 hours. It was 24 hours for few cases that contained more cortical bone due to the oblique direction when inserting biopsy-needle.
