Table 4.
Comparison of the immunohistochemical results
| Item | EDTA vs HCl |
RDO vs HCl |
||
|---|---|---|---|---|
| EDTA | HCl | RDO | HCl | |
| HER2/CEP17 SISH | 5/5 (100)a | 0/5 (0) | 0/5 (0) | 0/5 (0) |
| Kappa ISH | 7/7 (100) | 4/7 (57.1) | 2/3 (66.7) | 2/3 (66.7) |
| Lambda ISH | 5/5 (100) | 1/5 (20) | 2/2 (100) | 0/2 (0) |
| CyclinD1 | 9/9 (100) | 2/9 (22.2) | 3/3 (100) | 1/3 (33.3) |
| Ki67 | 9/9 (100) | 5/9 (55.6) | 10/10 (100) | 6/10 (45.5) |
| Bcl2 | 1/1 (100) | 1/1 (100) | 4/4 (100) | 4/4 (100) |
| Bcl6 | No data | No data | 1/1 (100) | 1/1 (100) |
| TdT | No data | No data | 1/1 (100) | 0/1 (0) |
| CD138 | 7/7 (100) | 7/7 (100) | 3/3 (100) | 3/3 (100) |
| CD20 | 9/9 (100) | 9/9 (100) | 9/9 (100) | 9/9 (100) |
| CD79a (B cell) | No data | No data | 2/2 (100) | 2/2 (100) |
| CD3 | 5/5 (100) | 5/5 (100) | 4/4 (100) | 4/4 (100) |
| CD5 | No data | No data | 3/3 (100) | 3/3 (100) |
| CD23 | No data | No data | 1/1 (100) | 1/1 (100) |
| CD10 | 1/1 (100) | 1/1 (100) | 1/1 (100) | 1/1 (100) |
| CD30 | 1/1 (100) | 1/1 (100) | No data | No data |
| Myeloperoxidase | 1/1 (100) | 1/1 (100) | 2/2 (100) | 2/2 (100) |
HCl, hydrochloric acid; EDTA, ethylenediaminetetraacetic acid disodium salt dehydrate; RDO, RDO GOLD; SISH, silver in situ hybridization; ISH, in situ hybridization.
a
Case number showing intact stain result among overall case number stained with each item (%). Positive expression in the indicated tumor cells or internal controls was considered as intact stain. For example, positive expression of cyclin D1 in mantle cell lymphoma cells or endothelial cells was interpreted as intact stain result.