Inhibition of PAI-1 prevents wound closure and re-epithelialization. Paraffin sections (5 μm) of control (A) and tiplaxtinin-treated (B, C), 5 days postinjury, wounds were stained with H&E 5 days after wounding. Images were taken with a 10× (left; dashed box indicates location of magnified image) and 20× (right) objective (D, dermis; E, epidermis). (D) Quantitation of percent wound closure as described in the Materials and Methods section (data are presented as mean±SEM; n=5 in duplicate; *p<0.05). (E) Quantitation of re-epithelialization (data are presented as mean±SEM; n=5 in duplicate; *p<0.05). H&E, hematoxylin and eosin; PAI-1, plasminogen activator inhibitor-1; TPX, tiplaxtinin.