Figure 3.

Tiplaxtinin has no effect on keratinocyte growth but inhibits fibroblast proliferation. (A) Subconfluent HaCaT cells were treated with 10 μM tiplaxtinin for 72 h. Cells were collected, stained with propidium iodide, and DNA content analyzed through FACS. (B) Paraffin sections (5 μm) of control and tiplaxtinin-treated wounds were stained with Ki-67 5 days after wounding. Images were taken with a 20× objective (D, dermis; E, epidermis, wound edge is indicated by arrows). (C, D) Quantitation of Ki-67-positive keratinocytes (C) versus fibroblasts (D) was calculated by direct cell counts in three different fields (data are presented as mean±SEM; *p<0.05).