Figure 5.

Tiplaxtinin does not alter wound site keratinocyte or fibroblast apoptotic index. Paraffin sections (5 μm) of control (A) and tiplaxtinin-treated (B) wounds were stained with TUNEL 5 days after wounding. In (A) and (B), arrows indicate TUNEL-positive apoptotic cells (C) No terminal deoxynucleotide transferase served as a negative control. (D) DNase treatment was used as a positive control. Images were taken with a 10× (left; dashed box indicates location of magnified image) and 20× (right) objective (D, dermis; E, epidermis). (E) Subconfluent HaCaT were pretreated with 10 μM tiplaxtinin or vehicle control for 24 h, as indicated. Cells were collected, stained with caspase-3, and analyzed by FACS. TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.