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. 2015 Jun 1;25(3):113–120. doi: 10.1089/nat.2014.0527

FIG. 4.

FIG. 4.

Ago2 expression affects modulation of splicing by ss-siRNA. (A) Purified cell cytoplasmic or nuclear extract reveals Ago2 in cell nuclei. Calreticulin is a marker for endoplasmic reticulum. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a marker for cytoplasm. Lamin A/C, RNA polymerase 2, and histone H3 are nuclear markers. ER, endoplasmic reticulum. (B) Knockdown of Ago2 mRNA in DMD patient fibroblast GM03429 using anti-Ago2 siRNA (50 nM) compared with the effect of treating cells with noncomplementary RNA duplex CM. (C) Effect of silencing Ago2 on modulation of splicing by ss-siRNA (100 nm) or an antisense oligonucleotide. Patient-derived GM03429 fibroblast cells were used.