Table 3.

Silencing RNAs Used to Test Effect of Ago2 Expression on Modulation of Splicing by ss-siRNA

Name	Antisense sequence (5′ to 3′)	ss or dsRNA	Target gene	Position of mismatch (if any)
CM	5′-GCUAUACCAGCGUCGUCAUdTdT	ds	Negative control
642661	5′-P-sCsAAsGGsAAsGUsUGsGCsAsUsUsUsCss	ss	Exon 51, DMD	10
678870	5′-UsCsAsAsGsGsAsAsGsAsUsGsGsCsAsUsUsUsCsU	ss, 2′-O-Me	Exon 51, DMD	-

Modification positions within the sequence are indicated as follows: boxed, 2′-O-MOE (methoxyethyl); underlined, 2′-Fluoro; background shaded, 2′-O-me (methyl); bold and italicized, mismatched bases; subscripted “s,” phosphorothioate (PS) linkage; d, deoxyribose. DMD, Duchenne's muscular dystrophy; dsRNA, double stranded RNA; ssRNA, single-stranded RNA.