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Instability of ARS plasmids with the two minichromosomal origins oriC6 and oriC7. Transformants with pOC1, pOC2, pOC6, pOC7, and pOP were propagated in nonselective AS168 broth for two passages. At each passage, the cultures were plated on AS168 medium, and the colonies were patched on AS168 (Mev) selective plates to determine the fraction of mevinolin-resistant cells.
