Figure 1. Common pathway for TORC2 and Myc regulated cell growth.

(A) Size comparisons between wild type (w¹¹¹⁸) and lst8¹, dm^P0, lst8¹ dm^P0, or rictor^Δ1 flies. One-day-old male flies were used for the analysis. (B) Quantification of animal weights. Genotypes of one-day-old male flies are shown. Asterisks indicate statistically significant differences (Student’s unpaired t-test, **p < 0.01; ***p < 0.001; n.s., not significant). (C–F) Comparisons between lst8¹ dm^P0, lst8¹, and dm^P0 fat body cells. (C) The FLP/FRT system was used to generate mosaic lst8¹, dm^P0, or lst8¹ dm^P0 mutant clones marked by the absence of RFP (hs-flp ub-RFP FRT/lst8¹ FRT, hs-flp ub-RFP FRT/dm^P0 FRT, and hs-flp ub-RFP FRT/lst8¹ dm^P0 FRT animals, respectively) (red, RFP; green, phalloidin; blue, DAPI). (D) Relative size of lst8¹, dm^P0, or lst8¹ dm^P0 cells compared with controls. Asterisks indicate statistically significant differences from wild-type cells (Student’s unpaired t-test, ***p < 0.001; n.s., not significant). (E) The FLP/FRT system was used to generate mosaic lst8¹ dm^P0 double mutant clones in larval fat bodies of lst8¹ hs-flp ub-RFP FRT/lst8¹ dm^P0 FRT and dm^P0 hs-flp ub-RFP FRT/lst8¹ dm^P0 FRT animals, respectively. The lst8¹ dm^P0 mutant cells are marked by the absence of RFP (red, RFP; green, Phalloidin; blue, DAPI). (F) Relative sizes of lst8¹ dm^P0 double mutant cells compared with lst8¹ or dm^P0 cells. Asterisks indicate statistically significant differences from single mutant cells (Student’s unpaired t-test, **p < 0.01; n.s., not significant). (G and H) Flow cytometry was performed on dissociated wing discs from lst8¹ hs-flp ub-RFP FRT/lst8¹ dm^P0 FRT and dm^P0 hs-flp ub-RFP FRT/lst8¹ dm^P0 FRT animals. Cells lacking lst8 (lst8¹/lst8¹; green trace in G), dm (dm^P0/dm^P0; green trace in H), or lst8 and dm (lst8¹ dm^P0/lst8¹ dm^P0; red trace) were compared. Hoechst 33342 staining was used to assess DNA content (right), and FSC was used to quantify cell size (left).