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. 2015 May 22;5:10535. doi: 10.1038/srep10535

Figure 4. Minocycline did not rescue synaptic and dendritic loss in Dicer cKO mice.

Figure 4

(A) Western blotting for SVP38 and PSD95. Cortical samples of 4 groups of mice were used. β–actin served as the internal control. (B) Quantitative results for SVP38. There was significant difference in SVP38 levels between control and cKO mice (p < 0.05). There was no difference in SVP38 levels between minocyline- and saline-treated Dicer cKO mice (p > 0.1). (C)Quantitative results for PSD95. There was significant difference in PSD95 levels between control and cKO mice (p < 0.05). However, there was no difference in PSD95 levels between minocyline- and saline- treated Dicer cKO mice (p > 0.1). (D) Immunohistochemistry for SVP38. Significantly reduced SVP38 immuno-reactivity was found in Dicer cKO mice (c,d,g,h), as compared to control mice (a,b,e,f). There was no difference in SVP38 immuno-reactivity between minocyline- and saline-treated cKO mice. (E) Immunohistochemistry for MAP2. Compared to control mice (a,b,e,f), Dicer cKO (c,d,g,h) exhibited significantly decreased MAP2 immuno-reactivity in the cortex and the hippocampus. However, there was no difference in MAP2 immuno-reactivity between minocyline- and saline-treated Dicer cKO mice. Scale bar = 25μm. Raw Western blotting images for SVP38 and PSD95 were shown in Supplementary Figures 5-6.