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. 2015 May 22;5:9795. doi: 10.1038/srep09795

Figure 3.

Figure 3

The expression levels of survivin (-WT and -2B) in RA-FLS are upregulated by PDGF, but not by IL-1β, TNF-α or leptin.(a) Cultured primary synoviocytes from RA patients were analyzed for survivin-WT and -2B expressions using a Western blot analysis. The survivin expression levels in the adherent cultured primary synoviocytes decreased with the cells passages. The cropped blots were used in the figure. (b) PDGF increased the survivin-WT and -2B expression levels, as indicated by a Western blot analysis. The cropped blots were used in the figure. (c–g) RA-FLS (n = 3 from different RA individuals) were cultured in the presence of (c) PDGF (10 ng/ml), (e) IL-1β (1 ng/ml), (f) TNF-α (20 ng/ml) or (g) leptin (1000 nM) for 24 to 48 hours and (d) PDGF (from 0.1 to 10.0 ng/ml) for 48 hours, after serum starvation (0.1%FBS/DMEM) for 24 hours. The time- and dose-dependent relative expression levels of survivin-WT and -2B were analyzed by real-time PCR. The relative expression levels were corrected based on the level of HPRT. Adherent FLS were used for experiments (without (a)) at passages 3 through 6. The results are the Means ± SEM (standard error of the mean). The Dunnett method, ∗∗p < 0.01, ∗p < 0.05.